BS EN ISO 20418-3 pdf free download

BS EN ISO 20418-3-2020 pdf free download.Textiles – Qualitative and quantitative proteomic analysis of some animal hair fibres – Part 3: Peptide detection using LC-MS without protein reduction (ISO 20418-3:2020).
This document specifies a qualitative and quantitative procedure to determine the composition of animal hair fibre blends (made of wool, cashmere, yak, alpaca, camel or angora) by LC-MS without protein reduction.
NOTE 1 The composition of non-animal hair fibres can be measured by ISO 1833 (all parts). Both results are combined to determine the total fibre composition.
The method is based on a preliminary identification, by light microscopy, of all fibres in the blend on the basis of their morphology, according to ISO/TR 11827 [4] . It is not applicable if fibres of the same animal species (such as blends of cashmere and mohair) are present.
NOTE 2 In this case, the quantitative analysis is performed using microscopical analysis [for example,
ISO 17751 (all parts)].
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content constitutes requirements of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.
ISO 1833-1, Textiles — Quantitative chemical analysis — Part 1: General principles of testing
ISO 3696, Water for analytical laboratory use — Specification and test methods
ISO 17751 (all parts), Textiles — Quantitative analysis of cashmere, wool, other specialty animal fibers and their blends
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
ISO and IEC maintain terminological databases for use in standardization at the following addresses:
— ISO Online browsing platform: available at https:// www .org/ obp
— IEC Electropedia: available at http:// www .electropedia .org/
3.1 animal hair fibre
type of keratin fibre for textile use, such as wool, cashmere, yak, alpaca, camel or angora
3.2 Bovidae
biological family of cloven-hoofed, ruminant mammals including cashmere goat, sheep and yak
3.3 Camelidae
biological family of even-toed ungulate mammals including camel and alpaca
3.4 protein
polymer of amino acids that play many critical roles in the body
3.5 peptide
small protein (3.4) consisting of approximately less than 50 amino acids
3.6 marker peptide
portion of a protein (3.4) used for its identification, recovery and purification
3.7 mass chromatogram
chromatogram for a specific mass-to-charge ratio
3.8 total ion chromatogram
TIC
chromatogram with each data point created by summing up intensities of all mass spectral peaks belonging to the same scan
3.9 selected ion monitoring SIM
mass spectrometry scanning mode in which only a limited m/z range is transmitted/detected by the instrument
4 Symbols and abbreviated terms
A peak area
W blending ratio
Br Bovidae rate
Cr Camelidae rate
ka correction factor for alpaca and camel
kc correction factor for cashmere
kr correction factor for angora
ky correction factor for yak
m/z mass to charge ratio, where m is the mass, expressed in atomic mass unit, and z is the charge
number of ions
5 Principle
The mechanically powdered fibres are directly subjected to trypsin digestion without prior reduction.
The analysis of the digested peptides is performed with LC-MS. The percent composition is calculated from the peak areas of the species-specific marker peptides.BS EN ISO 20418-3 pdf download.