BS EN 71-12 pdf free download

BS EN 71-12 2016 pdf free download.Safety of toys Part 1 2: N-Nitrosamines and N-nitrosatable substances.
5 Principle
N-nitrosamines and N-nitrosatable substances migrate into a test solution. N-nitrosatable substances are converted to N-nitrosamines by acidification. The final test solutions are analysed for N-nitrosamines by a suitable validated analytical technique. The N-nitrosamines and N-nitrosatable substances are expressed in mg/kg of sample.
The test solution for N-nitrosamines in finger paints is water. The test solution for N-nitrosatable substances in finger paints and for N-nitrosamines and N-nitrosatable substances in elastomers is saliva test solution.
6 Reagents and apparatus
6.1 Reagents
Unless otherwise specified, all reagents shall be of analytical grade and distilled water, or water of equivalent purity conforming to at least grade 3 of EN ISO 3696.
6.1.1 Sodium hydrogen carbonate (CAS 144-55-8)
6.1.2 Sodium chloride (CAS 7647-14-5)
6.1.3 Potassium carbonate (CAS 584-08-7)
6.1.4 Sodium nitrite (CAS 7632-00-0)
6.1.5 Hydrochloric acid (CAS 7647-01-0), aqueous solution, c(HCL) = 0,1 mol/l
6.1.6 Sodium hydroxide (CAS 1310-73-2), aqueous solution, c(NaOH) = 0,1 mol/l
6.1.7 Saliva test solution
Dissolve (4,2 ± 0,021) g of the sodium hydrogen carbonate (6.1.1), (0,5 ± 0,002 5) g of the sodium chloride (6.1.2), (0,2 ± 0,001 0) g of the potassium carbonate (6.1.3) and (0,03 ± 0,000 15) g of the sodium nitrite (6.1.4) in water and dilute to (950 ± 5) ml with water. Adjust to pH 9,0 ± 0,1 if necessary by adding the hydrochloric acid solution (6.1.5) or sodium hydroxide solution (6.1.6) drop by drop.
Transfer into a 1 l volumetric flask and dilute to the mark with water.
This solution has a limited stability. Saliva test solution kept for more than five days shall not be used.
6.1.8 Hydrochloric acid solution, c(HCL) = 1 mol/l.
6.1.9 Sodium hydroxide solution, c(NaOH) = 1 mol/l.
6.1.10 Methanol, ≥ 99,9 %, HPLC-grade.
6.1.11 Formic acid, ≥ 99,5 %, LC-MS grade.
6.1.12 Eluent A: 0,1 % (volume fraction) formic acid in water.
6.1.13 Eluent B: 0,1 % (volume fraction) formic acid in methanol.
6.2 Standards
N-nitrosamines are degraded by ultraviolet light. Exposure of extracts or standards to sources such as sunlight or fluorescent tube light should be avoided. The standards shall be protected by wrapping in aluminium foil or using amber glassware and stored in the dark at a temperature of (5 ± 3) °C.
WARNING — N-nitrosamines can endanger human health owing to their toxicity. Persons using this
European Standard should be familiar with normal laboratory practice. This European Standard does not purport to address all of the safety problems, if any, associated with its use. It is the responsibility of the user to establish appropriate safety and health practices and to ensure compliance with any national regulatory conditions.
6.2.1 N-nitrosamines or their solutions, with known purities.
6.2.2 Internal standard, d8-N-nitrosodiethanolamine (d8-NDELA), with known purity.
6.2.3 Internal standard, d6-N-nitrosodimethylamine (d6-NDMA), with known purity.
6.2.4 Stock solution of N-nitrosamines
Prepare a stock solution containing 10 mg/l of each N-nitrosamine (6.2.1) in methanol (6.1.10). This solution shall be stored in the absence of light at (−18 ± 3) °C.
NOTE Certified N-nitrosamines standards can be purchased from several suppliers including storage and stability information.
6.2.5 Solutions of internal standards
6.2.5.1 Stock solution of internal standards for elastomers
Prepare a stock solution containing 1 mg/l d8-NDELA (6.2.2) and 1 mg/l d6-NDMA (6.2.3) in methanol
(6.1.10). This solution shall be stored in the absence of light at (−18 ± 3) °C.
6.2.5.2 Working solution of internal standards for finger paints Prepare an internal standard working solution containing 1 mg/l d8-NDELA (6.2.2) in water. This solution has a stability of one day. 6.2.6 Calibration solutions
6.2.6.1 Calibration solutions for N-nitrosamines in finger paints Prepare five calibration solutions by diluting the stock solution of the N-nitrosamines (6.2.4) and the working solution of d8-NDELA (6.2.5.2) with water. The concentrations of the calibration solutions shall be 0,001 mg/l, 0,002 mg/l, 0,005 mg/l, 0,010 mg/l and 0,020 mg/l. Each solution shall contain 0,010 mg/l of d8-NDELA. These solutions have a stability of one day.BS EN 71-12 pdf download.