BS EN 1276 pdf free download
BS EN 1276-2019 pdf free download.Chemical disinfectants and antiseptics — Quantitative suspension test for the evaluation of bactericidal activity of chemical disinfectants and antiseptics used in food, industrial, domestic and institutional areas — Test method and requirements (phase 2, step 1).
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content constitutes requirements of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. EN 12353, Chemical disinfectants and antiseptics – Preservation of test organisms used for the determination of bactericidal (including Legionella), mycobactericidal, sporicidal, fungicidal and virucidal (including bacteriophages) activity EN 14885:2018, Chemical disinfectants and antiseptics – Application of European Standards for chemical disinfectants and antiseptics
3 Terms and definitions
For the purposes of this document, the terms and definitions given in EN 14885:2018 apply.
ISO and IEC maintain terminological databases for use in standardization at the following addresses:
• IEC Electropedia: available at http://www.electropedia.org/
• ISO Online browsing platform: available at https://www.iso.org/obp
4 Requirements The product shall demonstrate at least a 5 decimal logarithm (lg) reduction (3 lg for handwashes) when diluted with hard water (5.2.2.7) or – in the case of ready-to-use products – with water (5.2.2.2) and tested in accordance with Clause 5 under simulated clean conditions (0,3 g/l bovine albumin solution- 5.2.2.8.2) or simulated dirty conditions (3 g/l bovine albumin solution – 5.2.2.8.3) according to its practical applications and under the suitable test conditions as described in 5.5.1.1, Tables 1 and 2 here below.
Where indicated, additional specific bactericidal activity shall be determined applying other interfering substances and test organisms (in accordance with 5.2.1, 5.2.2.8 and 5.5.1.1) in order to take into account intended specific use conditions.
5 Test method
5.1 Principle
5.1.1 A sample of the product as delivered and/or diluted with hard water (or water for ready-to-use products with the exception of handwash products whose first dilution is done in hard water (5.4.2)) is added to a test suspension of bacteria in a solution of an interfering substance. The mixture is maintained at the chosen test temperature for the adopted contact time. At the end of this contact time, an aliquot is taken, and the bactericidal and/or the bacteriostatic activity in this portion is immediately neutralized or suppressed by a validated method. The method of choice is dilution-neutralization. If a suitable neutralizer cannot be found, membrane filtration is used. The numbers of surviving bacteria in each sample are determined and the reduction is calculated.
5.1.2 The test is performed using Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus and Enterococcus hirae as test organisms. For temperatures ≥ 40 °C only Enterococcus faecium shall be used.
For testing of hand hygiene products, Pseudomonas aeruginosa, Escherichia coli K12, Staphylococcus aureus and Enterococcus hirae are used as test organisms.
5.1.3 Additional test organisms can be used.
5.2 Materials and reagents
5.2.1 Test organisms
The bactericidal activity shall be evaluated using the following strains as test organisms:
— Pseudomonas aeruginosa ATCC 15442;
— Escherichia coli ATCC 10536;
— Staphylococcus aureus ATCC 6538;
— Enterococcus hirae
ATCC 10541;
— Escherichia coli K12
NCTC 10538;
— Enterococcus faecium ATCC 6057.
If required for specific applications, additional strains may be chosen, for example from:
— Salmonella Typhimurium ATCC 13311;
— Lactobacillus brevis DSM 6235;
— Enterobacter cloacae DSM 6234.BS EN 1276 pdf download.
